Bimolecular Fluorescence Complementation

Title
Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells

Title
Lighting-Up Tau-Tau Interaction in Living Cells
Authors
탁혜진마무눌 하크김민정이주현백자현김영수김동진Regis Grailhe김윤경
Keywords
tau; BiFC; interaction; oligomerization; real time
Issue Date
2013-12
Publisher
PLoS ONE
Citation
VOL 8, NO 12, e81862-1-e81862-7
Abstract
Abnormal tau aggregation is a pathological hallmark of many neurodegenerative disorders and it is becoming apparent that soluble tau aggregates play a key role in neurodegeneration and memory impairment. Despite this pathological importance, there is currently no single method that allows monitoring soluble tau species in living cells. In this regard, we developed a cell-based sensor that visualizes tau self-assembly. By introducing bimolecular fluorescence complementation (BiFC) technique to tau, we were able to achieve spatial and temporal resolution of tau-tau interactions in a range of states, from soluble dimers to large aggregates. Under basal conditions, tau-BiFC cells exhibited little fluorescence intensity, implying that the majority of tau molecules exist as monomers. Upon chemically induced tau hyperphosphorylation, BiFC fluorescence greatly increased, indicating an increased level of tau-tau interactions. As an indicator of tau assembly, our BiFC sensor would be a useful tool for investigating tau pathology.
URI
http://pubs.kist.re.kr/handle/201004/46944
ISSN
19326203
Appears in Collections:
KIST Publication > Article
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