Simultaneous analysis of urinary phthalate metabolites of residents in Korea using isotope dilution gas chromatography-mass spectrometry
- Simultaneous analysis of urinary phthalate metabolites of residents in Korea using isotope dilution gas chromatography-mass spectrometry
- 김미옥; 송나래; 최종호; 이정애; 표희수
- Urinary phthalate metabolites; Gas chromatography-mass spectrometry; Trimethylsilylation; Isotope dilution; Biomonitoring
- Issue Date
- The Science of the total environment
- VOL 470-471, 1408-1413
- Phthalates are used in industry products, household items, and medical tools as plasticizers. Human exposure to
phthalates has raised concern about its toxicity. In the present study, optimization was conducted for the simultaneous
analysis of eight kinds of phthalate metabolites using gas chromatography–mass spectrometry (GC-MS):
MEP, MiBP, MnBP, MBzP, MiNP, MEHP, MEOHP, and MEHHP. In order to minimize the matrix effect and to do
quantitative analysis, isotope dilution and LLE-GC-MS methods were performed. Urine samples were enzymatically
hydrolyzed, extracted with a mixture of n-hexane and ethyl ether (8:2; v:v), and subsequently derivatized
with trimethylsilylation. All eight kinds of analytes showed clear resolution and high reproducibility in GC-MS results.
The method detection limit ranged from 0.05 ng/mL to 0.2 ng/mL. Calibration curves were found to be linear
from 0.2 to 100 ng/mL with –2 > 0.992. The relative standard deviation of the intraday precision using water
and urine ranged from 2.1% to 16.3%. The analysis was performed with urine samples that were collected from
adults residing in the Republic of Korea. The analyzed concentration results were compared according to gender
and region. As a result, DEHP metabolites showed the highest detected concentration (75.92 μg/g creatinine,
100%), and MiNP, a metabolite of DiNP, showed the lowest detected concentration (0.42 μg/g creatinine,
22.5%). On average, female urine (200.76 μg/g creatinine) had a higher detected concentration of Σ8 phthalate
metabolites thanmale urine. Samples fromrural regions (211.96 μg/g creatinine) had higher levels than samples
from urban regions.
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