Both targeted mass spectrometry and flow sorting analysis methods detected the decreased serum apolipoprotein e level in Alzheimer's disease patients

Title
Both targeted mass spectrometry and flow sorting analysis methods detected the decreased serum apolipoprotein e level in Alzheimer's disease patients
Authors
한선호김준석이영주최희선김종원나덕률양은경유명희황대희이철주묵인희
Issue Date
2014-02
Publisher
Molecular & cellular proteomics : MCP
Citation
VOL 13, NO 2, 407-419
Abstract
Apolipoprotein E (ApoE) polymorphism has been appreciated as a valuable predictor of Alzheimer disease (AD), and the associated ε4 allele has been recognized as an indicator of susceptibility to this disease. However, serum ApoE levels have been a controversial issue in AD, due to the great variability regarding the different target detection methods, ethnicity, and the geographic variations of cohorts. The aim of this study was to validate serum ApoE levels in relation to AD, particularly using two distinct detection methods, liquid chromatography.selected reaction monitoring (SRM) mass spectrometry and microsphere- based fluorescence-activated cell sorting (FACS) analysis, to overcome experimental variations. Also, comparison of serum ApoE levels was performed between the level of protein detection by FACS and peptide level by SRM in both control and AD patients. Results from the two detection methods were cross-confirmed and validated. Both methods produced fairly consistent results, showing a significant decrease of serum ApoE levels in AD patients relative to those of a control cohort (43 control versus 45 AD, p < 0.0001). Significant correlation has been revealed between results from FACS and SRM (p < 0.0001) even though lower serum ApoE concentration values were measured in protein by FACS analysis than in peptidelevel detections by SRM. Correlation study suggested that a decrease of the serum ApoE level in AD is related to the mini-mental state exam score in both results from different experimental methods, but it failed to show consistent correlation with age, gender, or clinical dementia rating.
URI
http://pubs.kist.re.kr/handle/201004/47267
ISSN
15359476
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE