Measurement of mechanical property of Rat Brain Cells by Atomic Force Microscope

Measurement of mechanical property of Rat Brain Cells by Atomic Force Microscope
이상명Thi Huong Nguyen윤의성
Brain cell; cell stiffness; AFM; glia
Issue Date
Biofrontier Symposium 2012
Recently, atomic force microscopy (AFM) has become a powerful methodology for studying the biophysical properties (such as stiffness, viscoelasticity, hardness and adhesion). The unique combination of high-resolution imaging and operation in physiological environment made it useful in investigations of cell properties. A change in the physical properties, in particular cell elasticity, of tissue cells has been recognized as an indication of disease and has emerged as a marker for cellular phenotypic events associated with cell adhesion and cytoskeletal organization. In this study, using atomic force microscopy, we measured the stiffness of rat brain cells including normal cells (microglia) and cancer cells (glioblastoma). Two types of microglia cells extracted from the brain tissue of one-day-old- and eight-week-old-rats and glioblastoma cells (about eight-week-old-rats) cultured from C6 cell line were applied to the stiffness measurement. The cells were commonly attached on the mica surface coated with fibronectin and gelatin by incubating for 4 days. The measurement of cell stiffness was performed using the silicon cantilever with the rectangular shape (0.06 N/m). The working speed of the cantilever was 30 nm/sec. As a result, first, the microglias from adult rat (~ 100 kPa) were much harder than those from the pup rat (~10 kPa). This result enables us estimate the stiffness of the microglia cells according to the aging although ultimate two data were presented. Second, the glioblastoma (~ 50 kPa) are more than ~ 50% softer than normal cells. This correspond to the result that there is a reduction in stiffness with increasing metastatic efficiency in human cancer cell lines using several different in vitro biomechanical assays [1]. In conclusion, we demonstrated that the usefulness of nanomechanical analyses for the estimation of the aging of cells and the detection of the cancer cell, which is able to substitute t
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