Biomonitoring of urinary DEHP metabolites and its biomarkers based on Envirometabolomics

Biomonitoring of urinary DEHP metabolites and its biomarkers based on Envirometabolomics
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Di(2-ethylhexyl) phthalate (DEHP) is one of the common phthalate plasticizers used primarily in soft polyvinyl chloride including personal-care products, industrial plastics, and medical devices. The DEHP is rapidly metabolized to its monoester, mono(2-ethylhexyl)phthalate (MEHP), which is further metabolized by various hydroxylation and oxidation to the secondary metabolites such as mono(2-ethyl-5- hydroxyhexyl)phthalate (5OH-MEHP) and mono(2-ethyl-5-oxo-hexyl)phthalate (5-oxo- MEHP). Recently, it is known that DEHP causes adverse effects on endocrine functions and reproductive organs of humans. The objectives of this study were to examine the excretion of urinary DEHP metabolites and to assess human health risk from DEHP exposure. Trimethylsilylation (TMS) was employed and derivatives were analyzed using isotope dilution gas chromatography-mass spectrometry(GC-MS). The geometric mean (GM) levels of DEHP metabolites concentration tended to decrease with the increase of age, had the highest level was a group of 3-4 years (923.36 pmol/mg creatinine). Relative metabolite ratio (RMR) were calculated to examine the difference in DEHP exposure levels to the primary and secondary metabolites. MEHP hydroxylation to 5-OH-MEHP (1ststep ,RMR1) followed by 5-OH-MEHP oxidation to 5-oxo-MEHP(2ndstep, RMR2). In the urinary excretion ratio of DEHP metabolites by ages, RMR1 tended to decrease with the increase of age. RMR2 has a relatively constant value. RMRt also had similar patterns with RMR1. But RMRt has a higher value than RMR1. It seems to be used as potential biomarkers that can be used as a representative value according to age.
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