Investigation of cellulolytic bacteria Bacillus sp. SPF35 and Paenibacillus sp. CAA11 for consolidated bioprocessing
- Investigation of cellulolytic bacteria Bacillus sp. SPF35 and Paenibacillus sp. CAA11 for consolidated bioprocessing
- 김은숙; 강금래; 우한민; 엄영순
- cellulose; cellulase
- Issue Date
- Cellulosic biomass is the most abundant renewable bio-resource for the production of bio-fuel and bio-chemicals. Recent studies focus on consolidated bioprocessing (CBP) for hydrolyzing cellulose and fermentation with a single microbe. Most of CBP studies have developed in non-cellulolytic microorganisms such as E. coli, yeast, Z. mobilis which are widely developed for ethanol and bio-chemical production. In this study, natural cellulolytic bacteria Bacillus sp. SPF35 and Paenibacillus sp. CAA11 were investigated for bio-chemical production through CBP. Bacillus sp. SPF35 and Paenibacillus sp. CAA11 were isolated from soil of Indonesia and Dongmak foreshore in Korea. These strains secreted cellulase by own secretion system and produced lactic acid, formic acid, acetic acid and ethanol. To enhance cellulase activity, a strong promoter was screened using GFP gene as a reporter gene. Application of the strong promoter in cellulase overexpression resulted in 2-fold bigger halo zone on cellulose plate and bio-chemical production also increased under anaerobic culture condition. These results suggest the good potential of two cellulolytic strains for CBP in bio-fuel and chemical production.
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