Adding values to biodiesel-derived glycerol: High production of 2,3-butanediol using engineered Klebsiella oxytoca M1
- Adding values to biodiesel-derived glycerol: High production of 2,3-butanediol using engineered Klebsiella oxytoca M1
- 조숙형; 김태연; 우한민; 엄영순
- 2,3-butanediol; Klebsiella oxytoca; biodiesel-derived glycerol
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- Biodiesel production generates a great amount of waste glycerol equivalent to 10 % of biodiesel weight produced. Conversion of this waste glycerol into valuable products not only increases industrial profits but also reduces waste disposal cost. Several Klebsiella species have been studied to produce 2,3-butanediol (2,3-BDO) from glycerol. However, in previous studies, 1,3-propanediol (1,3-PDO) was also produced considerably. In this study, we report high production of 2,3-BDO from biodiesel-derived glycerol by reducing by-products such as 1,3-PDO and lactic acid using engineered K. oxytoca M1 (ΔdhaBΔldhA). The mutant strain produced 2-fold more 2,3-BDO than that of wild type in fed-batch from pure glycerol. Furthermore, using biodiesel-derived glycerol as a sole carbon source, the mutant showed similar glycerol consumption rate and growth rate comparing with pure glycerol culture in fed-batch fermentation, implying no inhibitory effect of waste glycerol. As a result, 2,3-BDO titer reached 131.5 g/L with the mutant, which was 13.5 % higher than that using pure glycerol. To the best of our knowledge, this result is the highest 2,3-BDO production from biodiesel-derived glycerol.
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