Investigation of Microbial Lignin Utilization: Lignin Enhanced the Growth of Bacillus sp. GWD275

Title
Investigation of Microbial Lignin Utilization: Lignin Enhanced the Growth of Bacillus sp. GWD275
Authors
공경택우한민엄영순박태현
Issue Date
2014-04
Publisher
2014년춘계생물공학회
Abstract
The lignin which is the most abundant natural aromatic polymer is well known to be recalcitrant for biodegradation and toxic to bacterial cells. Previously, Bacillus sp. GWD275 was isolated from mud flat thorough Azure B decolorization screening method for lignin degradation. In this study, lignin utilization by Bacillus sp. GWD275 was investigated by observing growth enhancement in the presence of lignin. The growth of GWD275 was compared with Luria-Bertani (LB) and LB supplemented with lignin (1 g/L). The growth in LB supplemented with lignin was increased by 1.8-fold compared with LB medium only, implying lignin utilization by GWD275. In addition, when defined medium with glucose or xylose without complex nitrogen source was used, the growth of GWD275 was also enhanced in the presence of lignin (0.5 g/L), possibly by using lignin as a co-substrate for growth. The results presenting here would provide an important opportunity for efficient use of limited lignocellulosic bio-resources by lignin utilization using Bacillus sp. GWD275.
URI
http://pubs.kist.re.kr/handle/201004/47469
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KIST Publication > Conference Paper
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