Investigation of Microbial Lignin Utilization: Lignin Enhanced the Growth of Bacillus sp. GWD275
- Investigation of Microbial Lignin Utilization: Lignin Enhanced the Growth of Bacillus sp. GWD275
- 공경택; 우한민; 엄영순; 박태현
- Issue Date
- The lignin which is the most abundant natural aromatic polymer is well known to be recalcitrant for biodegradation and toxic to bacterial cells. Previously, Bacillus sp. GWD275 was isolated from mud flat thorough Azure B decolorization screening method for lignin degradation. In this study, lignin utilization by Bacillus sp. GWD275 was investigated by observing growth enhancement in the presence of lignin. The growth of GWD275 was compared with Luria-Bertani (LB) and LB supplemented with lignin (1 g/L). The growth in LB supplemented with lignin was increased by 1.8-fold compared with LB medium only, implying lignin utilization by GWD275. In addition, when defined medium with glucose or xylose without complex nitrogen source was used, the growth of GWD275 was also enhanced in the presence of lignin (0.5 g/L), possibly by using lignin as a co-substrate for growth. The results presenting here would provide an important opportunity for efficient use of limited lignocellulosic bio-resources by lignin utilization using Bacillus sp. GWD275.
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