Application of a novel enzymatic pretreatment using crude hydrolytic extracellular enzyme solution to microalgal biomass for dark fermentative hydrogen production
- Application of a novel enzymatic pretreatment using crude hydrolytic extracellular enzyme solution to microalgal biomass for dark fermentative hydrogen production
- 윤여명; 김동훈; 오유관; 신항식; 정경원
- Chlorella Vulgaris; Food waste; Crude hydrolytic extracellular enzyme solution; Co-substrate
- Issue Date
- Bioresource technology
- VOL 159, 365-372
- In this study, a novel enzymatic pretreatment of Chlorella vulgaris for dark fermentative hydrogen production
(DFHP) was performed using crude hydrolytic extracellular enzyme solution (CHEES) extracted from
the H2 fermented effluent of food waste. It was found that the enzyme extracted at 52 h had the highest
hydrolysis efficiency of microalgal biomass, resulting in the highest H2 yield of 43.1 mL H2/g dry cell
weight along with shorter lag periods. Even though a high amount of VFAs was accumulated in CHEES,
especially butyrate, the fermentative bacteria on the DFHP was not affected from product inhibition. It
also appears that the presence of organic acids, especially lactate and acetate, contained in the CHEES
facilitated enhancement of H2 production acted as a co-substrate. Therefore, all of the experimental
results suggest that the enhancement of DFHP performance caused by CHEES has a dual role as the hydrolysis
enhancer and the co-substrate supplier.
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