Cellobiose utilization in Corynebacterium glutamicum by in vivo direct evolution
- Cellobiose utilization in Corynebacterium glutamicum by in vivo direct evolution
- 이정석; 엄영순; 오민규; 우한민
- C. glutamicum; cellobiose; metabolic engineering; direct evolution
- Issue Date
- 2014 한국생물공학회 춘계학술발표대회 및 국제심포지엄
- Corynebacterium glutamicum is a widely-known industrial bacterium for amino acid and varieties of biochemical. C. glutmaicum wild type is able to utilize glucose but not able to utilize cellobiose as sole carbon source. We have engineered C. glutamicum for utilization of cellobiose, which are major carbon sources. Cellobiose is made from hydrolyzed biomass with endo- and exo-cellulsase without beta-glucosidase to reduced usage of cellulases and reducing the cost of hydrolysis. In this study, Corynebacterium glutamicum was engineered by introducing cellobiose utilization pathway constructed in the BioBrick-formatted expression vector systems. The cellobiose utilization pathway consists of transporter and β-glucosidases from other microorganisms. Heterologous target genes were synthetized and optimized via in vivo direct evolution. The resulting C. glutamicum strains were capable of utilizing cellobiose as sole carbon source and reached the same optical density of glucose-consuming wild-type within 12 hr. The strains will be further applied to industrial strains to produce variety of chemicals. This work was supported by the ‘Creative Allied Program (CAP)’ through the Korea Research Council of Fundamental Science and Technology (KRCF) and Korea Institute of Science and Technology (KIST).
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