Systems metabolic engineering to overcome the toxicity of a hydrolysate inhibitor, Furfural in Corynebacterium glutamicum
- Systems metabolic engineering to overcome the toxicity of a hydrolysate inhibitor, Furfural in Corynebacterium glutamicum
- 박홍실; 엄영순; 심상준; 우한민
- lignocellulosic hydrolysate; furfural; Corynebacterium glutamicum
- Issue Date
- 2014 한국생물공학회 춘계학술발표대회 및 국제심포지엄
- Pretreatment of lignocellulosic biomasses by acid hydrolysis generates cellular inhibitors such as Furfural, HMF, etc. This by-product formation reduced the productivity of target chemicals. Therefore, it is necessary to overcome the toxicity in the hydrolysate. We focused the effect of the inhibitors to Corynebacterium glutamicum, which is widely used for industrial strains. Transcriptome analysis of C. glutamicum grown in presence of furfural was investigated, resulting in levels of 50 % genes changed. In order to identify the target genes for gene amplification, genes belonging to the functional categories were chosen by several criteria. We have engineered C. glutamicum by overexpressing the target genes encoding for reductase and efflux-permease and cultivated the engineered strains with furfural. They were shown to be furfural tolerant. This target genes could be further applied to construct the microbial cell factories. This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government (MEST) (2014, University-Institute cooperation program).
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