Inhibitory Effects of Ferula ferulaeoides and Carduus crispus on Allergy-Related Mediator Production in Rat Basophilic Leukemia Mast Cells
- Inhibitory Effects of Ferula ferulaeoides and Carduus crispus on Allergy-Related Mediator Production in Rat Basophilic Leukemia Mast Cells
- 임수지; 김명석; 노주원
- Ferula ferulaeoides; RBL-2H3; Carduus crispus; IgE; Degranulation
- Issue Date
- 생화학분자생물학회 (KSBMB)
- Ferula ferulaeoides (Steud.) Korov., an important species of the genus Ferula, has a long history of the application in treatment of spasm, digestive diseases and arthritis. Carduus crispus L. (Asteraceae) is mainly found in Asia and China and has been used in traditional medicine for the treatment of colds, stomach aches, and rheumatism for a long time. However, effects of Ferula ferulaeoides extract (FFE) and Carduus crispus extract (CCE) on immunoglobulin (Ig) E-mediated allergic responses still remain elusive. In the present study, the effects of FFE and CCE on activation and degranulation of rat basophilic leukemia (RBL-2H3) mast cells were investigated. To evaluate the antiallergic effects of FFE and CCE, we determined its effects on degranulation, and inflammatory mediators in IgE-stimulated RBL-2H3 cells. FFE and CCE inhibited secretion of β-hexosaminidase and histamine (FFE only). FFE and CCE reduced the production of prostaglandin E2 and interleukin-4 and the mRNA expression of interleukin-4 and tumor necrosis factor-α. Furthermore, FFE and CCE attenuated phosphorylation of the mitogen-activated protein kinases extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase expression. These results indicate that FFE and CCE might be useful as an agent against immediate-type hypersensitivity.
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