Aurantio-obtusin Isolated from Cassia tora decreases oleic acid-induced lipid accumulation via AMPK phosphorylation in hepatocarcinoma cells
- Aurantio-obtusin Isolated from Cassia tora decreases oleic acid-induced lipid accumulation via AMPK phosphorylation in hepatocarcinoma cells
- 김명석; 임수지; 노주원
- Aurantio-obtusin; Cassia tora; AMPK; HepG2; Lipogenesis; Lipolysis
- Issue Date
- 생화학분자생물학회 (KSBMB)
- Anthraquinone constituents isolated from the seeds of Cassia tora have been reported to possess antifungal, antioxidant, liver protective, and hypolipidemic activities. The present study was aimed to investigate the protective effects of aurantio-obtusin isolated from Cassia tora on hepatocyte cultured with high fatty acid and the underlying mechanisms. By using human hepatoma cell HepG2 as cell model, the results showed that aurantio-obtusin suppressed fatty acid synthesis and enhanced fatty acid oxidation, contributing to amelioration of lipid accumulation induced by oleic acid. Moreover, aurantio-obtusin also stimulated adenosine monophosphate-activated protein kinase. Aurantio-obtusin attenuated the expression of sterol regulatory element-binding protein-1 and its target molecules, such as fatty acid synthase. In contrast, the lipolytic enzyme expressions of peroxisome proliferator activated receptor α and carnitinepalmitol- transferase-1 were increased. This study suggests the hypolipidemic effects of aurantio-obtusin occur via phosphorylation of AMPK and inhibition of lipid biosynthesis and stimulation of lipolysis. Therefore, the aurantio-obtusin may actively prevent nonalcoholic fatty liver disease.
- Appears in Collections:
- KIST Publication > Conference Paper
- Files in This Item:
There are no files associated with this item.
- RIS (EndNote)
- XLS (Excel)
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.