Aurantio-obtusin Isolated from Cassia tora decreases oleic acid-induced lipid accumulation via AMPK phosphorylation in hepatocarcinoma cells

Title
Aurantio-obtusin Isolated from Cassia tora decreases oleic acid-induced lipid accumulation via AMPK phosphorylation in hepatocarcinoma cells
Authors
김명석임수지노주원
Keywords
Aurantio-obtusin; Cassia tora; AMPK; HepG2; Lipogenesis; Lipolysis
Issue Date
2014-05
Publisher
생화학분자생물학회 (KSBMB)
Abstract
Anthraquinone constituents isolated from the seeds of Cassia tora have been reported to possess antifungal, antioxidant, liver protective, and hypolipidemic activities. The present study was aimed to investigate the protective effects of aurantio-obtusin isolated from Cassia tora on hepatocyte cultured with high fatty acid and the underlying mechanisms. By using human hepatoma cell HepG2 as cell model, the results showed that aurantio-obtusin suppressed fatty acid synthesis and enhanced fatty acid oxidation, contributing to amelioration of lipid accumulation induced by oleic acid. Moreover, aurantio-obtusin also stimulated adenosine monophosphate-activated protein kinase. Aurantio-obtusin attenuated the expression of sterol regulatory element-binding protein-1 and its target molecules, such as fatty acid synthase. In contrast, the lipolytic enzyme expressions of peroxisome proliferator activated receptor α and carnitinepalmitol- transferase-1 were increased. This study suggests the hypolipidemic effects of aurantio-obtusin occur via phosphorylation of AMPK and inhibition of lipid biosynthesis and stimulation of lipolysis. Therefore, the aurantio-obtusin may actively prevent nonalcoholic fatty liver disease.
URI
http://pubs.kist.re.kr/handle/201004/47585
Appears in Collections:
KIST Publication > Conference Paper
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