Ginsenoside Rg3 Enhances Islet Cell Function and Attenuates Apoptosis in Mouse Islets
- Ginsenoside Rg3 Enhances Islet Cell Function and Attenuates Apoptosis in Mouse Islets
- S.S. Kim; Hyuk Jai Jang; M.Y. Oh; D.W. Eom; K.S. Kang; 김영주; 이지환; 함정엽; S.Y. Choi; Y.M. Wee; Y.H. Kim; D.J. Han
- Ginsenoside Rg3; Enhance Islet
Cell Function; Transplantation; Antiapoptosis
- Issue Date
- Transplantation proceedings
- VOL 46, NO 4, 1150-1155
- Background. The transplantation of isolated islets is thought to be an attractive approach
for curative treatment of diabetes mellitus. Panax ginseng has been used in oriental
countries for its pharmacologic effects, such as antidiabetic and antiinflammatory activities.
20(S)-ginsenoside Rg3 (Rg3), an active ingredient of ginseng saponins, has been reported
to enhance insulin secretionestimulating and antiapoptotic activities in pancreatic beta
cells. We performed this study to examine the hypothesis that preoperative Rg3 administration
can enhance islet cell function and antiapoptosis before islet transplantation.
Methods. Balb/c mice were randomly divided into 2 groups according to the administration
of Rg3 after islet isolation. Mouse islets were cultured in medium supplemented
with or without Rg3. In vitro, islet viability and function were assessed. After treatment of
islets with a cytokine cocktail (tumor necrosis factor α, interferon-γ, and interleukin-1β),
cell viability, function, and apoptosis were assessed.
Results. Cell viability was similar between the 2 groups. Islets cultured in medium supplemented
with Rg3 showed 2.3-fold higher glucose-induced insulin secretion than islets
cultured in medium without Rg3. After treatment with a cytokine cocktail, glucoseinduced
insulin release, total insulin content of islets, and apoptosis were significantly
improved in Rg3-treated islets compared with cytokine-treated islets. Cytokine-treated
islets produced significantly higher levels of nitric oxide (NO) than islets treated with Rg3.
Conclusions. These results suggest that preoperative Rg3 administration enhanced islet
function before islet transplantation and attenuated both cytokine-induced damage associated
with NO production and apoptosis. Rg3 administration might be a prospective management
to enhanced islet function and ameliorate early inflammation after transplantation.
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