DNA translocation in two dimensional nanopore
- DNA translocation in two dimensional nanopore
- 맹준호; 손근식; 차병준; 한상희; 신현준; 이상엽
- nanopore; friction matrix; polyethylene glycol; 나노포어; 마찰발생구조물; 폴리에틸렌 글라이콜
- Issue Date
- 대한기계학회 마이크로/나노 분과
- Nanopores that can transport ions and molecules are promising devices for single-molecule sensing and genomic screening. In DNA sequencing, however, efforts to minimize read error such as decreasing the DNA translocation velocity through the pore and full-stretching the DNA molecules have been critical issues. We present a friction matrix-based DNA translocation velocity reduction and DNA pre-extension using an addition of a functional polymer. A nanofluidic device consisting of two dimensional (2D) nanopores and tapered nanochannels was fabricated using standard electron beam lithography and anodic bonding method in silicon and glass plate. 2D Nanopore arrays with two area ratios (α = 0.15 and 0.18) of nanochannel to nanopore were also fabricated through the size-shrinkage using 150 nm thermal oxidation. We investigated the dynamics of electrophoretic translocation of λ-DNA molecules in TBE buffer (pH 8.03) with and without 1 % polyethylene glycol (PEG, Mw = 10K) solution served as polymer material for friction matrix. As a result, friction matrix by polymer network was not only decreasing DNA translocation velocity through the pore by over 37% but increasing the length of the stretched DNA by over 15% compared with TBE buffer without the polymer. We believe that the proposed method will be simple and useful for DNA analysis in nanofluidic devices.
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