Sophoraflavanone G Isolated from Sophora flavescens decreases oleic acid-induced lipid accumulation via AMPK phosphorylation in hepatocarcinoma cells

Title
Sophoraflavanone G Isolated from Sophora flavescens decreases oleic acid-induced lipid accumulation via AMPK phosphorylation in hepatocarcinoma cells
Authors
김명석이희주임수지노주원
Keywords
Sophora flavescens; Sophoraflavanone G; AMPK; HepG2; triglyceride
Issue Date
2014-06
Publisher
2014년도 한국응용생명화학회 국제학술대회
Abstract
Prenylated flavonoids isolated from the seeds of Sophora flavescens have been reported to possess antbacterial, antioxidant, anti-inflammation, and anti-allergic activities. The present study was aimed to investigate the protective effects of Sophoraflavanone G (SFG) isolated from S. flavescens extract (SFE)on hepatocyte cultured with high fatty acid and the underlying mechanisms. By using human hepatoma cell HepG2 as cell model, the results showed that SFE and SFG suppressed fatty acid synthesis and enhanced fatty acid oxidation, contributing to amelioration of lipid accumulation induced by oleic acid. Moreover, SFE and SFG also stimulated adenosine monophosphate-activated protein kinase. SFE and SFG attenuated the expression of sterol regulatory element-binding protein-1 and its target molecules, such as fatty acid synthase. In contrast, the lipolytic enzyme expressions of peroxisome proliferator activated receptor α and carnitinepalmitol- transferase-1 were increased. This study suggests the hypolipidemic effects of SFE and SFG occur via phosphorylation of AMPK and inhibition of lipid biosynthesis and stimulation of lipolysis. Therefore, the SFE and SFG may actively prevent nonalcoholic fatty liver disease.
URI
http://pubs.kist.re.kr/handle/201004/47737
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KIST Publication > Conference Paper
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