Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling

Title
Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling
Authors
Ki-Young Chang우도연Hyunjin JungSangkyu LeeSungsoo Kim원종하Taeyoon KyungHyerim ParkNury KimHee Won YangJae-Yong Park황은미Daesoo KimWon Do Heo
Keywords
Biological sciences; Bioengineering; Cell biology
Issue Date
2014-06
Publisher
Nature Communications
Citation
VOL 5, 4057-1-4057-10
Abstract
Receptor tyrosine kinases (RTKs) are a family of cell-surface receptors that have a key role in regulating critical cellular processes. Here, to understand and precisely control RTK signalling, we report the development of a genetically encoded, photoactivatable Trk (tropomyosinrelated kinase) family of RTKs using a light-responsive module based on Arabidopsis thaliana cryptochrome 2. Blue-light stimulation (488 nm) of mammalian cells harbouring these receptors robustly upregulates canonical Trk signalling. A single light stimulus triggers transient signalling activation, which is reversibly tuned by repetitive delivery of blue-light pulses. In addition, the light-provoked process is induced in a spatially restricted and cell-specific manner. A prolonged patterned illumination causes sustained activation of extracellular signal-regulated kinase and promotes neurite outgrowth in a neuronal cell line, and induces filopodia formation in rat hippocampal neurons. These light-controllable receptors are expected to create experimental opportunities to spatiotemporally manipulate many biological processes both in vitro and in vivo.
URI
http://pubs.kist.re.kr/handle/201004/47766
ISSN
20411723
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KIST Publication > Article
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