DNA amplification in neutral liposomes for safe and efficient gene delivery

Title
DNA amplification in neutral liposomes for safe and efficient gene delivery
Authors
이상민구희범나진희이경은정서영최귀원김선화권익찬김광명
Keywords
polymerase chain reaction; neutral liposomes; gene delivery; nanofactory
Issue Date
2014-05
Publisher
ACS Nano
Citation
VOL 8, NO 5, 4257-4267
Abstract
In general, traditional gene carriers contain strong cationic charges to efficiently load anionic genes, but this cationic character also leads to destabilization of plasma membranes and causes severe cytotoxicity. Here, we developed a PCR-based nanofactory as a safe gene delivery system. A few template plasmid DNA can be amplified by PCR inside liposomes about 200 nm in diameter, and the quantity of loaded genes highly increased by more than 8.8-fold. The liposome membrane was composed of neutral lipids free from cationic charges. Consequently, this system is nontoxic, unlike other traditional cationic gene carriers. Intense red fluorescent protein (RFP) expression in CHO-K1 cells showed that the amplified genes could be successfully transfected to cells. Animal experiments with the luciferase gene also showed in vivo gene expression by our system without toxicity. We think that this PCR-based nanofactory system can overcome the toxicity problem that is the critical limitation of current gene delivery to clinical application.
URI
http://pubs.kist.re.kr/handle/201004/47793
ISSN
19360851
Appears in Collections:
KIST Publication > Article
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