DNA amplification in neutral liposomes for safe and efficient gene delivery
- DNA amplification in neutral liposomes for safe and efficient gene delivery
- 이상민; 구희범; 나진희; 이경은; 정서영; 최귀원; 김선화; 권익찬; 김광명
- polymerase chain reaction; neutral liposomes; gene delivery; nanofactory
- Issue Date
- ACS Nano
- VOL 8, NO 5, 4257-4267
- In general, traditional gene carriers contain strong cationic charges to efficiently load anionic genes, but this cationic character also leads to destabilization of plasma membranes and causes severe cytotoxicity. Here, we developed a PCR-based nanofactory as a safe gene delivery system. A few template plasmid DNA can be amplified by PCR inside liposomes about 200 nm in diameter, and the quantity of loaded genes highly increased by more than 8.8-fold. The liposome membrane was composed of neutral lipids free from cationic charges. Consequently, this system is nontoxic, unlike other traditional cationic gene carriers. Intense red fluorescent protein (RFP) expression in CHO-K1 cells showed that the amplified genes could be successfully transfected to cells. Animal experiments with the luciferase gene also showed in vivo gene expression by our system without toxicity. We think that this PCR-based nanofactory system can overcome the toxicity problem that is the critical limitation of current gene delivery to clinical application.
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