Phytoestrogenic activity of Aceriphyllum rossii and rapid identification of phytoestrogens by LC-NMR/MS and bioassay-guided isolation

Title
Phytoestrogenic activity of Aceriphyllum rossii and rapid identification of phytoestrogens by LC-NMR/MS and bioassay-guided isolation
Authors
강석우강경수김미애전나라김상민전제승노주원엄병헌
Keywords
Aceriphyllum rossii; Phytoestrogenic activity; LC-NMR/MS; Quercetin; Kaempferol
Issue Date
2014-08
Publisher
European food research and technology; Zeitschrift fü r Lebensmittel-Untersuchung und -Forschung. A
Citation
VOL 239, NO 2, 237-246
Abstract
Aceriphyllum rossii Engler (Saxifragaceae) has been used as a nutritious food in Korea, but only minimal studies on this plant have been undertaken. We have examined the estrogenic effect of A. rossii and have discovered its potent constituents. To identify the estrogenic activity of A. rossii, we measured the transactivation of the estrogen-responsive element (ERE) in MCF-7 cells transfected with an ERE-containing construct after treatment of the extract and fractions of A. rossii. A. rossii showed a potent estrogenic activity and gallic acid ethyl ester; quercetin and kaempferol were identified as major constituents of the active fractions by liquid chromatography–nuclear magnetic resonance spectroscopy/mass spectrometry. We confirmed quercetin and kaempferol are the major active constituents after activity-guided isolation of seven compounds: gallic acid ethyl ester (1), quercetin (2), kaempferol (3), quercetin-3-O-(6″-galloyl)-β-d-glucopyranoside (4), quercetin-3-O-β-d-glucopyranoside (5), kaempferol-3-O-(6″-galloyl)-β-d-glucopyranoside (6), and kaempferol-3-O-β-d-glucopyranoside (7). These results show A. rossii could be a possible candidate for the treatment of postmenopausal symptoms.
URI
http://pubs.kist.re.kr/handle/201004/47856
ISSN
14382377
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KIST Publication > Article
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