Interdigitated Microelectrode Sensor for Blood-based Early Diagnosis of Alzheimer’s Disease

Interdigitated Microelectrode Sensor for Blood-based Early Diagnosis of Alzheimer’s Disease
Issue Date
Biosensors 2014
Alzheimer’s disease (AD) constitutes one of the biggest threats to public health. Current medical AD diagnosis depends on interviews and the molecular neuroimaging. However, diagnosis from interviews is not accurate. In spite of neuroimaging analysis method give a good help for medical verification, this is unsuitable to AD early diagnosis due to poor accessibility and high cost. Therefore, efforts to develop biomarkers in the blood for AD early diagnosis have been performed. One of the strong candidate biomarkers, beta-amyloid (Aβ), have a spotlight as AD biomarker which can detect AD in early stage. Because the concentration range of Aβ in blood is extremely low level (pg/mL), development of highly sensitive sensor platform strongly demanded. Here we developed the interdigitated microelectrodes (IMEs) sensor to detect the Aβ in blood. By monitoring the impedance change of surface functionalized IMEs, we could detect Aβ protein in real-time. The different and important point of our IMEs sensors compared with conventional intedigitated impedance sensor was functionalization region. While other intedigitated impedance sensor immobilized receptors on the intedigitated electrodes, we functionalized gap between neighbourhood IMEs by Aβ antibodies to improve the sensitivity. We fabricated platinum IMEs with width of 3~5 μm and length of 300 μm on the SiO2/Si substrate. The gap with 3~5 μm was functionalized self-assembled monolayers and Aβ antibodies. Impedance of IME sensors was measured by impedance analyzer. When the Aβ diluted solution with concentration of 1, 10, 100, and 1000 pg/mL was injected in reaction chamber with IME sensors, reactance of IMEs decreased 16.494, 17.395, 19.638, and 24.071 kohm, respectively. Moreover, reactance of nonspecific antibodies immobilized IMEs sensor decreased under 1 kohm. It indicated that sensitivity of our IME sensors was better than conventional method.
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