3'-Deoxyadenosine inhibits pre-adipocyte differentiation and biosynthesis of triacylglycerol in 3T3-L1 cells

Title
3'-Deoxyadenosine inhibits pre-adipocyte differentiation and biosynthesis of triacylglycerol in 3T3-L1 cells
Authors
신용규Michael B. Ye김성원이수찬김일웅김수남양현옥김시관
Keywords
3T3-L1 cells; in vitro pharmacokinetics; Cordycepin; Differentiation; Triacylglycerol; Anti-obesity
Issue Date
2014-01
Publisher
Journal of Functional Foods
Citation
VOL 6, 331-338
Abstract
This study intended to develop anti-obesity compounds from natural sources. In our screening program for anti-obesity agents, a compound isolated from Cordyceps militaris was found to be a potent inhibitor of the differentiation of 3T3-L1 pre-adipocytes. The active ingredient of C. militaris was isolated from methanol extracts by chromatography, re-crystallized in water, and subsequently identified as 30-deoxyadenosine, otherwise known as cordycepin. Cordycepin was a potent inhibitor of cellular differentiation and triacylglycerol (TAG) synthesis at a concentration of 32 lM without any signs of cytotoxicity. However, the inhibitory action was attenuated by supplementation with adenosine. Western blot analysis revealed that cordycepin down-regulated the protein levels of C/EBPa, PPAR-c, and anti-leptin; these proteins are known to be associated with TAG synthesis. Cordycepin-induced decrease in protein expression was countered by the addition of adenosine. In vitro pharmacokinetic studies demonstrate that 95% of the cordycepin (64 lM initial concentration) added to the culture medium of 3T3-L1 cells was cleared within 6 h. The metabolic rate of cordycepin in vitro was similar to that of adenosine, suggesting that cordycepin inhibits the differentiation of pre-adipocytes and blocks the synthesis of TAG in 3T3- L1 cells by interfering with adenosine metabolism.
URI
http://pubs.kist.re.kr/handle/201004/48039
ISSN
17564646
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KIST Publication > Article
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