3'-Deoxyadenosine inhibits pre-adipocyte differentiation and biosynthesis of triacylglycerol in 3T3-L1 cells
- 3'-Deoxyadenosine inhibits pre-adipocyte differentiation and biosynthesis of triacylglycerol in 3T3-L1 cells
- 신용규; Michael B. Ye; 김성원; 이수찬; 김일웅; 김수남; 양현옥; 김시관
- 3T3-L1 cells; in vitro pharmacokinetics; Cordycepin; Differentiation; Triacylglycerol; Anti-obesity
- Issue Date
- Journal of Functional Foods
- VOL 6, 331-338
- This study intended to develop anti-obesity compounds from natural sources. In our
screening program for anti-obesity agents, a compound isolated from Cordyceps militaris
was found to be a potent inhibitor of the differentiation of 3T3-L1 pre-adipocytes. The
active ingredient of C. militaris was isolated from methanol extracts by chromatography,
re-crystallized in water, and subsequently identified as 30-deoxyadenosine, otherwise
known as cordycepin. Cordycepin was a potent inhibitor of cellular differentiation and triacylglycerol (TAG) synthesis at a concentration of 32 lM without any signs of cytotoxicity.
However, the inhibitory action was attenuated by supplementation with adenosine. Western
blot analysis revealed that cordycepin down-regulated the protein levels of C/EBPa,
PPAR-c, and anti-leptin; these proteins are known to be associated with TAG synthesis.
Cordycepin-induced decrease in protein expression was countered by the addition of adenosine. In vitro pharmacokinetic studies demonstrate that 95% of the cordycepin (64 lM initial
concentration) added to the culture medium of 3T3-L1 cells was cleared within 6 h. The
metabolic rate of cordycepin in vitro was similar to that of adenosine, suggesting that cordycepin inhibits the differentiation of pre-adipocytes and blocks the synthesis of TAG in 3T3-
L1 cells by interfering with adenosine metabolism.
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