Biosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicum

Title
Biosynthesis of pinene from glucose using metabolically-engineered Corynebacterium glutamicum
Authors
강민경엄진희김연제엄영순우한민
Keywords
Corynebacterium glutamicum; pinene; Metabolic Engineering; Monoterpene; Toxicity
Issue Date
2014-10
Publisher
Biotechnology letters
Citation
VOL 36, NO 10, 2069-2077
Abstract
Pinene is a monoterpenes (C10) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. Herein, we have metabolically-engineered Corynebacterium glutamicum, to produce pinene and studied its toxicity in C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), obtained from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS (P. taeda) and GPPS (A. grandis) resulted in 27 μg ± 7 α-pinene g−1 cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain may increase pinene production.
URI
http://pubs.kist.re.kr/handle/201004/48089
ISSN
01415492
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE