The Immobilization of Bone Morphogenetic Protein-2 via Photo Curable Azidophenyl Hyaluronic Acid on a Titanium Surface and Providing Effect for Cell Differentiation

Title
The Immobilization of Bone Morphogenetic Protein-2 via Photo Curable Azidophenyl Hyaluronic Acid on a Titanium Surface and Providing Effect for Cell Differentiation
Authors
Hyung-Jae LeeYun HeoKyung-Tai ParkEun-Hye KimYoshihiro ItoKwang-Sup Song한동근Tae-Il Son
Keywords
titanium surface modification; osteoinductive effect; bone morphogenetic protein-2 (BMP-2); azidophenyl hyaluronic acid; photo immobilization; alkaline phosphatase (ALP) assay; alizarin red S staining
Issue Date
2014-02
Publisher
Macromolecular research
Citation
VOL 22, NO 2, 173-178
Abstract
Titanium and its alloys have been used for a wide range of dental implant materials because of their biocompatible properties. Due to the osseointegration between the bone and implant surface that is required after implantation surgery, surface modification of titanium has been investigated. Even though bone morphogenetic protein-2 (BMP-2) and other growth factors have been applied to increase the efficiency of osseointegration, their short half-life has been a disadvantage. In this study, BMP-2 was photo-immobilized on a titanium surface using azidophenyl hyaluronic acid (Az-HA) as a matrix, and C2C12 cell lines were cultured on the modified surface. The photoreactive azidophenyl hyaluronic acid was prepared and characterized in a previous report. To demonstrate the osteoinductive effect of the photo-immobilized BMPs, alkaline phosphatase (ALP) activity assay, calcium detection assay, and alizarin red staining were conducted.
URI
http://pubs.kist.re.kr/handle/201004/48136
ISSN
15985032
Appears in Collections:
KIST Publication > Article
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