Biosynthesis of pinene from glucose using metabolically engineered Corynebacterium glutamicum
- Biosynthesis of pinene from glucose using metabolically engineered Corynebacterium glutamicum
- 추승정; 엄영순; 한성옥; 우한민
- pinene; metabolic engineering
- Issue Date
- 2014 한국생물공학회 추계학술발표대회 및 국제심포지엄
- Pinene is a bicyclic monoterpene chemical compound (C10) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. In this study, to produce pinene and analyze its toxicity in Corynebacterium glutamicum, we use metabolically-engineered C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), derived from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS (P. taeda) and GPPS (A. grandis) resulted in 27 μg ± 7 α-pinene g−1 cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain are in progress to increase pinene production. This work was supported KIST Institutional program of the Korea Institute of Science and Technology (KIST).
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