Biosynthesis of pinene from glucose using metabolically engineered Corynebacterium glutamicum

Title
Biosynthesis of pinene from glucose using metabolically engineered Corynebacterium glutamicum
Authors
추승정엄영순한성옥우한민
Keywords
pinene; metabolic engineering
Issue Date
2014-10
Publisher
2014 한국생물공학회 추계학술발표대회 및 국제심포지엄
Abstract
Pinene is a bicyclic monoterpene chemical compound (C10) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. In this study, to produce pinene and analyze its toxicity in Corynebacterium glutamicum, we use metabolically-engineered C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), derived from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS (P. taeda) and GPPS (A. grandis) resulted in 27 μg ± 7 α-pinene g−1 cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain are in progress to increase pinene production. This work was supported KIST Institutional program of the Korea Institute of Science and Technology (KIST).
URI
http://pubs.kist.re.kr/handle/201004/48312
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KIST Publication > Conference Paper
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