Electricity-driven metabolic shift through direct electron uptake by electroactive heterotroph Clostridium pasteurianum

Title
Electricity-driven metabolic shift through direct electron uptake by electroactive heterotroph Clostridium pasteurianum
Authors
최옥경김태연우한민엄영순
Issue Date
2014-11
Publisher
Scientific Reports
Citation
VOL 4, 6961-1-6961-9
Abstract
Although microbes directly accepting electrons from a cathode have been applied for CO2 reduction to produce multicarbon-compounds, a high electron demand and low product concentration are critical limitations. Alternatively, the utilization of electrons as a co-reducing power during fermentation has been attempted, but there must be exogenous mediators due to the lack of an electroactive heterotroph. Here, we show that Clostridium pasteurianum DSM 525 simultaneously utilizes both cathode and substrate as electron donors through direct electron transfer. In a cathode compartment poised at 10.045 V vs. SHE, a metabolic shift in C. pasteurianum occurs toward NADH-consuming metabolite production such as butanol from glucose (20% shift in terms of NADH consumption) and 1,3-propandiol from glycerol (21% shift in terms of NADH consumption). Notably, a small amount of electron uptake significantly induces NADH-consuming pathways over the stoichiometric contribution of the electrons as reducing equivalents. Our results demonstrate a previously unknown electroactivity and metabolic shift in the biochemical-producing heterotroph, opening up the possibility of efficient and enhanced production of electron-dense metabolites using electricity.
URI
http://pubs.kist.re.kr/handle/201004/48503
ISSN
20452322
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