Development of lipidomic platform and phosphatidylcholine retention time index for lipid profiling of rosuvastatin treated human plasma
- Development of lipidomic platform and phosphatidylcholine retention time index for lipid profiling of rosuvastatin treated human plasma
- 최종민; 김태은; 조주연; 이화정; 정병화
- Lipidomics; UPLC-QTOF; Lipid; Rosuvastatin; Phosphatidylcholine
- Issue Date
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
- VOL 944, 157-165
- A simple and fast methodology to detect and identify multiple classes of lipid from human plasma is developed utilizing ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC–QTOF) as lipidomics platform. All the conditions for the sample preparation and analytical instruments were optimized in detail to detect nine lipid classes (phosphatidylserine (PS), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), triacylglyceride (TG), phosphatidylinositol (PI), lysophosphatidylcholine (LysoPC), lysophosphatidic acid (LysoPA), and sphingomyelin (SM)), which are the most important biologically active lipids but have different characteristics. Finally, the plasma was prepared after a liquid–liquid extraction with a mixture of chloroform/methanol (1:2 v/v) including salting out by adding 0.15 M of NaCl and the residue after evaporation was reconstituted with a mixture of chloroform/methanol (1:1 v/v) to dissolve all lipids which have different polarity. The chromatographic conditions were set up such that mobile phase (A) comprised 10 mM ammonium acetate in 40% acetonitrile and mobile phase (B) comprised 10 mM ammonium acetate in acetonitrile:isopropanol = 10:90 (v/v) with ACQUITY BEH C18 as the stationary phase. In particular, a retention time index of PC was constructed by analyzing known standards to confirm each variant of PC without the use of any additional standards in every experiment. The lipidomic methodology and the retention time index of PC were applied to analyze the lipidomic profiling of human plasma from rosuvastatin (lipid lowering drug) treated subjects.
In the developed lipidomic platform, all lipids were successfully analyzed within 16 min and PCs could be confirmed with the PC retention time index. In rosuvastatin treatment, the lipid profiling was changed in all the eight lipid classes. The level of SM, TG, PI and PE decrease
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