Neuroprotective effect of a traditional Korean herbal formula via autophagy induction in models of Parkinson's disease
- Neuroprotective effect of a traditional Korean herbal formula via autophagy induction in models of Parkinson's disease
- Nayoung Bae; Sungkwon Chung; 차진욱; Hyungun Oh; 김희주; 구밍야오; Myung Sook Oh; 양현옥
- Issue Date
- 62nd International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research
- Chungsimyeolda-tang, a traditional Korean herbal formula which contains 17 kinds of medicinal plants, has been used to treat cerebrovascular disease including stroke in Korea. In this study, we made efforts to explore if our modified Chungsimyeolda-tang (termed DG), containing three selected medicinal plants such as Angelica tenuissima Nakai, Polygala tenuifolia and Dimocarpus longan Lour from original prescription, has the possibility as a treatment for Parkinson’s disease (PD). Here, we verified the neuroprotective and autophagy inducing effects of DG. 1-methyl-4-phenylpyridinium (MPP+) was used to induce cytotoxicity in nerve growth factor (NGF)-differentiated rat pheochromocytoma (PC12) cells. Cell viability was measured using an MTT assay. Induction of autophagy by DG treatment up to 300 μg/ml concentration in NGF-differentiated PC12 cells was measured using an immunoblotting assay with an LC3 antibody. The proteasomal inhibitor lactacystin was used to induce ubiquitin-proteasome system dysfunction in NGF-differentiated PC12 cells. Clearance of aggregated proteins by DG was measured using an immunoblotting assay with an ubiquitin antibody. Our findings indicate that DG robustly protects NGF-differentiated PC12 cells against the neurotoxic effects of MPP+ in an in vitro model. Furthermore, DG provides neuroprotection against lactacystin-induced NGF-differentiated PC12 cell death. This effect is partially mediated by increased autophagy associated with enhanced degradation of aggregated proteins. These studies suggest that DG is an attractive candidate as an autophagy-inducing drug and may play a beneficial role in preventing diseases associated with
misfolded/aggregated proteins, including PD.
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