Hyaluronic Acid-siRNA Conjugate/Reducible Polyethylenimine Complexes for Targeted siRNA Delivery
- Hyaluronic Acid-siRNA Conjugate/Reducible Polyethylenimine Complexes for Targeted siRNA Delivery
- 장연림; 구숙희; 이소진; 박재형; 김원종; 권익찬; 김선화; 정지훈
- siRNA Delivery; Hyaluronic Acid; Reducible Polyethylenimine; Tumor Targeting
- Issue Date
- Journal of nanoscience and nanotechnology
- VOL 14, NO 10, 7388-7394
- The clinical applications of therapeutic siRNA remain as a challenge due to the lack of efficient
delivery system. In the present study, hyaluronic acid-siRNA conjugate (HA-SS-siRNA)/reducible polyethylenimine (BPEI1.2k-SS) complexes were developed to efficiently deliver the siRNA to HA receptor abundant region with the improved siRNA stability. HA and siRNA were conjugated with disulfide bonds, which are cleavable in cytoplasm. The synthesized HA-SS-siRNA was further complexed with BPEI1.2k-SS, resulting in the formation of spherical nanostructures with approximately 190 nm of size and neutral surface charge. HA-SS-siRNA/BPEI1.2k-SS complexes exhibited the improved stability against serum proteins or polyanions. These complexes were successfully translocated into intracellular region via HA receptor-mediated endocytosis, and silenced target gene expression.
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