Hyaluronic Acid-siRNA Conjugate/Reducible Polyethylenimine Complexes for Targeted siRNA Delivery

Title
Hyaluronic Acid-siRNA Conjugate/Reducible Polyethylenimine Complexes for Targeted siRNA Delivery
Authors
장연림구숙희이소진박재형김원종권익찬김선화정지훈
Keywords
siRNA Delivery; Hyaluronic Acid; Reducible Polyethylenimine; Tumor Targeting
Issue Date
2014-10
Publisher
Journal of nanoscience and nanotechnology
Citation
VOL 14, NO 10, 7388-7394
Abstract
The clinical applications of therapeutic siRNA remain as a challenge due to the lack of efficient delivery system. In the present study, hyaluronic acid-siRNA conjugate (HA-SS-siRNA)/reducible polyethylenimine (BPEI1.2k-SS) complexes were developed to efficiently deliver the siRNA to HA receptor abundant region with the improved siRNA stability. HA and siRNA were conjugated with disulfide bonds, which are cleavable in cytoplasm. The synthesized HA-SS-siRNA was further complexed with BPEI1.2k-SS, resulting in the formation of spherical nanostructures with approximately 190 nm of size and neutral surface charge. HA-SS-siRNA/BPEI1.2k-SS complexes exhibited the improved stability against serum proteins or polyanions. These complexes were successfully translocated into intracellular region via HA receptor-mediated endocytosis, and silenced target gene expression.
URI
http://pubs.kist.re.kr/handle/201004/48694
ISSN
15334880
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE