Stabilization of a hyaluronate-associated gene delivery system using calcium ions

Title
Stabilization of a hyaluronate-associated gene delivery system using calcium ions
Authors
Feng, MinIbrahim, Basma M.Wilson, Erin M.Doh, Kyung-OhBergman, Brandon K.Park, Christopher여윤
Issue Date
2014-02
Publisher
Biomaterials Science
Citation
VOL 2, 936-942
Abstract
A “DPH” ternary complex consisting of plasmid DNA (pDNA), intracellularly degradable polyethyleneimine, and hyaluronic acid (HA) is a promising non-viral gene carrier with low toxicity and good gene transfection efficiency. HA plays a key role in providing an optimal balance between DNA protection and release, but it causes aggregation due to the entanglement of HA chains of neighbouring DPH particles. Here we report that the addition of an optimal level of Ca2+ successfully prevents particle aggregation and maintains a relatively small size. The Ca-stabilized DPH is comparable to DPH in cytotoxicity and gene transfection efficiency. MW monitoring and conductometric titration suggest that such size stabilization effect is partly mediated by the complexation between HA and Ca2+, which enables intra- and intermolecular interactions of HAs.
URI
http://pubs.kist.re.kr/handle/201004/48740
ISSN
20474830
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE