Development of SPR biosensor for the detection of human hepatitis B virus using plasma-treated parylene-N film
- Development of SPR biosensor for the detection of human hepatitis B virus using plasma-treated parylene-N film
- Yong-Hwan Choi; Ga-Yeon Lee; Hyuk Ko; Young Wook Chang; 강민정; Jae-Chul Pyun
- Parylene N coating; SPR Sensor; Hepatitis Virus B; Parylene-N; Surface modification; Plasma; Immobilization; SPR biosensor
- Issue Date
- Biosensors and bioelectronics
- VOL 56, 286-294
- A plasma-treated parylene-N film was presented for the immobilization of proteins through physical adsorption. The changes in surface properties of the parylene-N film after plasma-treatment were analyzed using contact angle microscopy and AFM. To demonstrate the high protein-immobilization efficiency of the plasma-treated parylene-N film, the immobilization efficiencies of differently modified surfaces were compared using model proteins with different surface charges, such as streptavidin (pI=5, negatively charged at pH 7), horseradish peroxidase (pI=6.6, nearly neutral at pH 7), and avidin (pI=10, positively charged at pH 7). The application of the plasma-treated parylene-N film as an SPR biosensor was also tested by immobilizing model proteins. An SPR biosensor based on the plasma-treated parylene-N film was developed for the detection of the human hepatitis virus surface antigen (HBsAg), and the plasma-treated parylene-N film was estimated to improve the sensitivity of SPR biosensor as much as 1000-fold by enhancing immobilization of receptor antibodies.
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