Direct conversion of 5% (v/v) CO2 to acetone and isopropanol with metabolically-engineered cyanobacteria

Title
Direct conversion of 5% (v/v) CO2 to acetone and isopropanol with metabolically-engineered cyanobacteria
Authors
좌준원이현정최선영심상준우한민
Keywords
Cyanobacteria; Metabolic engineering; Synthetic biology; Acetone; Isopropanol; Ethanol; Environment
Issue Date
2014-11
Publisher
제3회 아시아-오세아니아 조류회담
Abstract
Cyanobacteria, photosynthetic prokaryotes, have been gathering global interests because of their ability of CO2 bio-mitigation. By metabolic engineering and synthetic biology, we tried to produce isopropanol from CO2 by metabolically engineering of Synechococcus elongatus PCC7942. First, we tested the toxicity of C2-C5 alcohols on the growth of S. elongatus PCC7942. Ethanol and isopropanol did not influence cellular growth but isobutanol and isopentanol. Subsequently, we constructed acetone and isopropanol-producing strains harboring heterologous acetone-isopropanol pathway using a synthetic platform of CyanoBrickTM for gene expression in S. elongatus PCC7942. The engineered strains were cultured under 5 % CO2 supply condition and isopropanol in the medium was analyzed by GC-FID. The strains produced small amount of acetone or isopropanol, compared to engineered E. coli strain. However, further metabolic engineering could be applied to enhance the titer of acetone and isopropanol in S. elongatus PCC7942.
URI
http://pubs.kist.re.kr/handle/201004/48794
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KIST Publication > Conference Paper
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