Direct conversion of 5% (v/v) CO2 to acetone and isopropanol with metabolically-engineered cyanobacteria
- Direct conversion of 5% (v/v) CO2 to acetone and isopropanol with metabolically-engineered cyanobacteria
- 좌준원; 이현정; 최선영; 심상준; 우한민
- Cyanobacteria; Metabolic engineering; Synthetic biology; Acetone; Isopropanol; Ethanol; Environment
- Issue Date
- 제3회 아시아-오세아니아 조류회담
- Cyanobacteria, photosynthetic prokaryotes, have been gathering global interests because of their ability of CO2 bio-mitigation. By metabolic engineering and synthetic biology, we tried to produce isopropanol from CO2 by metabolically engineering of Synechococcus elongatus PCC7942. First, we tested the toxicity of C2-C5 alcohols on the growth of S. elongatus PCC7942. Ethanol and isopropanol did not influence cellular growth but isobutanol and isopentanol. Subsequently, we constructed acetone and isopropanol-producing strains harboring heterologous acetone-isopropanol pathway using a synthetic platform of CyanoBrickTM for gene expression in S. elongatus PCC7942. The engineered strains were cultured under 5 % CO2 supply condition and isopropanol in the medium was analyzed by GC-FID. The strains produced small amount of acetone or isopropanol, compared to engineered E. coli strain. However, further metabolic engineering could be applied to enhance the titer of acetone and isopropanol in S. elongatus PCC7942.
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