Resveratrol Inhibits Glycine Receptor-Mediated Ion Currents

Title
Resveratrol Inhibits Glycine Receptor-Mediated Ion Currents
Authors
Byung-Hwan LeeSung-Hee HwangSun-Hye ChoiHyeon-Joong KimSeok-Won JungHyun-Sook KimJoon-Hee LeeHyung-Chun Kim임혜원Seung-Yeol Nah
Keywords
resveratrol; glycine; glycine receptor; ligand-gated ion channel; Xenopus oocyte
Issue Date
2014-04
Publisher
Biological & pharmaceutical bulletin
Citation
VOL 37, NO 4, 576-580
Abstract
Resveratrol is found in grapes, red wine, and berries. Resveratrol has been known to have many beneficial health effects, such as anti-cancer, neuroprotective, anti-nociceptive, and life-prolonging effects. However, the single cellular mechanisms by which resveratrol acts are relatively unknown, especially in terms of possible regulation of receptors involved in synaptic transmission. The glycine receptor is an inhibitory ligand-gated ion channel involved in fast synaptic transmission in spinal cord. In the present study, we investigated the effect of resveratrol on human glycine receptor channel activity. Glycine α1 receptors were expressed in Xenopus oocytes and glycine receptor channel activity was measured using a two-electrode voltage clamp technique. Treatment with resveratrol alone had no effect on oocytes injected with H2O or on oocytes injected with glycine α1 receptor cRNA. In the oocytes injected with glycine α1 receptor cRNA, co- or pretreatment of resveratrol with glycine inhibited the glycine-induced inward peak current (IGly) in a reversible manner. The inhibitory effect of resveratrol on IGly was also concentration dependent, voltage independent, and non-competitive. These results indicate that resveratrol regulates glycine receptor channel activity and that resveratrol-mediated regulation of glycine receptor channel activity is one of several cellular action mechanisms of resveratrol for pain regulation.
URI
http://pubs.kist.re.kr/handle/201004/49150
ISSN
09186158
Appears in Collections:
KIST Publication > Article
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