A new thin silicon microneedle with an embedded microchannel for deep brain drug infusion

Title
A new thin silicon microneedle with an embedded microchannel for deep brain drug infusion
Authors
이현주손유진김도희김윤경최낙원윤의성조일주
Keywords
Embedded microfluidic channel; Buried microchannel; Microneedle; Glass reflow
Issue Date
2015-03
Publisher
Sensors and actuators. B, Chemical
Citation
VOL 209, 413-422
Abstract
We present the implementation of a new thin silicon microneedle for deep brain drug infusion implemented using a fabrication technology called glass cover on silicon technology (GCoS) that embeds microchannels in silicon substrate. The embedded microchannels are formed by anodically bonding a glass wafer to a silicon wafer with cavities, reflowing glass to partially cover the top of the cavity, and removing the unwanted glass above the silicon substrate. Because no dielectric sealing process is required, long microchannels in mm range can be readily fabricated using GCoS and there is no restriction on the cross-sectional area or shape. In addition, the silicon substrate is directly available for further integrated circuit (IC) processing and consists of embedded microchannels that are transparent from top through the glass. Using GCoS, we have successfully implemented a microneedle designed for drug infusion with point targeting accuracy as well as less damage for small animal experiments. The fabricated microneedles were 5.3 mm in length and as small as 40 μm in thickness and 70 μm in width. Fluidic characterization with a pressure-driven injection system showed linearly decreasing flow rates ranging from 100 to < 50 nl/min. This linearity of the flow rates to input pressure confirms precise control of the low flow rates, which is important in injecting small quantity of drugs. After we verify successful infusions of trypan blue with our microneedles into both 0.9% w/v agarose gel and sacrificed mouse brain, we demonstrate the possibility of drug infusions with not only precise targeting capability but also less brain damage by infusing dyes in vivo followed by immunohistochemistry (IHC).
URI
http://pubs.kist.re.kr/handle/201004/49200
ISSN
09254005
Appears in Collections:
KIST Publication > Article
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