Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer

Title
Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer
Authors
성지혜Mingxing OuyangTaejin KimJie SunPo-Chao WenShaoying LuYue ZhuoNicholas M. LlewellynDavid D. SchlaepferJun-Lin GuanShu ChienYingxiao Wang
Issue Date
2011-07
Publisher
Nature Communications
Citation
VOL 2, NO 406, 1-9
Abstract
Proper subcellular localization of focal adhesion kinase (FAK) is crucial for many cellular processes. It remains, however, unclear how FAK activity is regulated at subcellular compartments. To visualize the FAK activity at different membrane microdomains, we develop a fluorescence resonance energy transfer (FRET)-based FAK biosensor, and target it into or outside of detergent-resistant membrane (DRM) regions at the plasma membrane. Here we show that, on cell adhesion to extracellular matrix proteins or stimulation by platelet-derived growth factor (PDGF), the FRET responses of DRM-targeting FAK biosensor are stronger than that at non-DRM regions, suggesting that FAK activation can occur at DRM microdomains. Further experiments reveal that the PDGF-induced FAK activation is mediated and maintained by Src activity, whereas FAK activation on cell adhesion is independent of, and in fact essential for the Src activation. Therefore, FAK is activated at membrane microdomains with distinct activation mechanisms in response to different physiological stimuli.
URI
http://pubs.kist.re.kr/handle/201004/49266
ISSN
20411723
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE