Gymnasterkoreayne B isolated from Gymnaster koraiensis Inhibits UVB-induced MMP Expression and Promotes Type-1 Procollagen Production through Nrf2 Activation in HaCaT Cells and Human Dermal fibroblasts
- Gymnasterkoreayne B isolated from Gymnaster koraiensis Inhibits UVB-induced MMP Expression and Promotes Type-1 Procollagen Production through Nrf2 Activation in HaCaT Cells and Human Dermal fibroblasts
- 남의정; 김명석; 임수지; 노주원
- Gymnasterkoreayne B; Gymnaster koraiensis; UVB; MMP; Type-1 Procollagen; Nrf2
- Issue Date
- 생화학분자생물학회 (KSBMB)
- Ultraviolet (UV) radiation damages human skin and causes skin diseases such as epidermal hyperplasia, sunburn, inflammatory responses and photoaging. Photoaging is associated with upregulated matrix metalloproteinase (MMP) expression and downregulated collagen synthesis. Gymnasterkoreayne B (GKB), which is isolated from Gymnaster koraiensis, has been reported to possess antioxidant and anticancer activities; however, its effects on photoaging are unknown. This study assessed the effects of GKB and extract of Gymnaster koraiensis (GKE) on photoaging and investigated its mechanisms of action in UVB-irradiated immortalized human keratinocytes (HaCaT) and human dermal fibroblast (HDF) by enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, Western blot analysis and 2′,7′-dichlorofluorescein diacetate assay. Our results showed that GKB and GKE attenuated UVB-induced MMP and inflammatory cytokine expression by deactivating NF-κB pathway induced by reactive oxygen species. GKB and GKE also increased type-I procollagen and antioxidant enzyme expression through nuclear factor-E2-related factor 2 (Nrf2) pathway. Taken together, GKB and GKE regulates the expression of MMPs and type-I procollagen in UVB-irradiated HaCaT cells and HDF by regulating NF-κB, and Nrf2, suggesting it as a potential candidate for prevention and treatment of skin aging.
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