Sorting of human mesenchymal stem cells by applying optimally designed microfluidic chip filtration

Title
Sorting of human mesenchymal stem cells by applying optimally designed microfluidic chip filtration
Authors
정희경전명석장미숙
Keywords
Cell sorting; Microfluidic chip; Hydrodynamic filtration; Stem cell; Channel network
Issue Date
2015-02
Publisher
Analyst
Citation
VOL 140, NO 4, 1265-1274
Abstract
Human bone marrow-derived mesenchymal stem cells (hMSCs) consist of heterogeneous subpopulations with different multipotent properties: small and large cells with high and low multipotency, respectively. Accordingly, sorting out a target subpopulation from the others is very important to increase the effectiveness of cell-based therapy. We performed flow-based sorting of hMSCs by using optimally designed microfluidic chips based on the hydrodynamic filtration (HDF) principle. The chip was designed with the parameters rigorously determined by the complete analysis of laminar flow for flow fraction and complicated networks of main and multi-branched channels for hMSCs sorting into three subpopulations: small (<25 μm), medium (25&#8211;40 μm), and large (>40 μm) cells. By focusing with a proper ratio between main and side flows, cells migrate toward the sidewall due to a virtual boundary of fluid layers and enter the branch channels. This opens the possibility of sorting stem cells rapidly without damage. Over 86% recovery was achieved for each population of cells with complete purity in small cells, but the sorting efficiency of cells is slightly lower than that of rigid model particles, due to the effect of cell deformation. Finally, we confirmed that our method could successfully fractionate the three subpopulations of hMSCs by analyzing the surface marker expressions of cells from each outlet.
URI
http://pubs.kist.re.kr/handle/201004/49297
ISSN
00032654
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE