In situ detoxification of lignocellulosic hydrolysate using a surfactant for butyric acid production by Clostridium tyrobutyricum ATCC 25755

Title
In situ detoxification of lignocellulosic hydrolysate using a surfactant for butyric acid production by Clostridium tyrobutyricum ATCC 25755
Authors
이경민김기연최옥경우한민김연제한성옥상병인엄영순
Keywords
butyric acid; lignocellulose; phenolic compound; in situ detoxification; tween 80
Issue Date
2015-04
Publisher
Process biochemistry
Citation
VOL 50, NO 4, 630-635
Abstract
Lignocellulosic degradation compounds, especially phenolic compounds, inhibit the fermentation of Clostridium strains. In this study, a simple in situ detoxification method using a surfactant was developed for butyric acid production by Clostridium tyrobutyricum ATCC25755. Tween 80, a non-ionic surfactant, was chosen to sequester inhibitors by forming micelles, consequently preventing direct contact of inhibitors with cell membranes. When Tween 80 was added during fermentation, butyric acid production was significantly enhanced in the presence of hydrophobic phenolics such as p-coumaric acid (0 vs. 3.1 g/L butyric acid) and ferulic acid (2.2 vs. 4.6 g/L butyric acid) even at 0.016 g/L of Tween 80 (corresponding to the critical micelle concentration). Lignin, a polyphenol compound in lignocellulose, was also detoxified by Tween 80. When Tween 80 was added to acid-pretreated rice straw hydrolysate, butyric acid production significantly increased (0.1 vs. 8.7 g/L butyric acid), thus verifying detoxification effect of Tween 80 on lignocellulosic hydrolysate.
URI
http://pubs.kist.re.kr/handle/201004/49527
ISSN
13595113
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KIST Publication > Article
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