Directed synthesis of bio-inorganic vanadium oxide composities using genetically modified filamentous phage
- Directed synthesis of bio-inorganic vanadium oxide composities using genetically modified filamentous phage
- Mueller, Michael; Baik, Seungyun; 전호정; 김유찬; Kim, Jungtae; Kim, Young Jun
- Filamentous phage; Vanadium oxides; nanocomposites; photodegradation; nanoelectronics
- Issue Date
- Applied surface science
- VOL 337, 12-18
- The growth of crystalline vanadium oxide using a filamentous bacteriophage template was investigatedusing sequential incubation in a V2O5precursor. Using the genetic modification of the bacteriophage,we displayed two cysteines that constrained the RSTB-1 peptide on the major coat protein P8, resultingin vanadium oxide crystallization. The phage-driven vanadium oxide crystals with different topologies,microstructures, photodegradation and vanadium oxide composites were characterized by scanning elec-tron microscopy (SEM), transmission electron microscopy (TEM), quartz microbalance and dissipation(QCM-D) and X-ray photoelectron spectroscopy (XPS). Non-specific electrostatic attraction between awild-type phage (wt-phage) and vanadium cations in the V2O5precursor caused phage agglomerationand fiber formation along the length of the viral scaffold. As a result, the addition of recombinant phage(re-phage) in V2O5precursors formed heterogeneous structures, which led to efficient condensationof vanadium oxide crystal formation in lines, shown by QCM-D analysis. Furthermore, re-phage/VxOxcomposites showed significantly enhanced photodegradation activities compared with the synthesizedwt-phage-V2O5composite under illumination. This study demonstrates that peptide-mediated vana-dium oxide mineralization is governed by a complicated interplay of peptide sequence, local structure,kinetics and the presence of a mineralizing aid, such as the two cysteine-constrained peptides on thephage surface, and has potential for use in nanotechnology applications.
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