MicroRNA sequencing detects up-regulation of miR-424-5p in ovarian cancer stem cells

Title
MicroRNA sequencing detects up-regulation of miR-424-5p in ovarian cancer stem cells
Authors
윤지호임지선하인수신지민김정훈김정호노주원조윤신
Keywords
MicroRNA; miR-424-5p; ovarian cancer stem cells
Issue Date
2015-05
Publisher
생화학분자생물학회 (KSBMB)
Abstract
Cancer stem cells (CSCs) are cancer cells that possess a capability of continuous proliferation and self-renewal. It has been believed that CSCs are responsible for tumor growth, heterogeneity, invasion, metastasis, and recurrence. MicroRNAs, a small non-coding RNAs containing about 22 nucleotides, have been known to be involved in the maintenance of CSCs. To gain insight into the role of microRNAs in CSCs, we investigated the differentially expressed microRNAs in ovarian CSCs compared to non-CSCs. Ovarian CSCs were isolated from the human ovarian cancer cell line SK-OV-3 using two ovarian CSC specific surface markers, CD44 and CD117. The expression levels of microRNAs in CSCs and non-CSCs were estimated by microRNA sequencing method. We were able to detect four up-regulated miRNAs (miR-29a-5p, -34c-5p, -106a-5p, and -424-5p) in ovarian CSCs, of which miR-424-5p was validated by real-time qPCR. The miR-424-5p target genes were predicted from several validated target databases as well as computational algorithms. Pathway analysis indicated that most miR-424-5p target genes are involved in cancer related biological pathways. In total, these results suggest that miR-424-5p is a potential regulator of CSCs endowing human ovarian tissue with the tumorigenesis, and the possible therapeutic target for human ovarian cancer. -
URI
http://pubs.kist.re.kr/handle/201004/49757
Appears in Collections:
KIST Publication > Conference Paper
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE