Use of composite protein database including search result sequences for mass spectrometric analysis of cell secretome

Title
Use of composite protein database including search result sequences for mass spectrometric analysis of cell secretome
Authors
신지혜김가민Kabir Mohammad Humayun박성준이승택이철주
Issue Date
2015-03
Publisher
PLoS ONE
Citation
VOL 10, NO 3, e0121692
Abstract
Mass spectrometric (MS) data of human cell secretomes are usually run through the conventional human database for identification. However, the search may result in false identifications due to contamination of the secretome with fetal bovine serum (FBS) proteins. To overcome this challenge, here we provide a composite protein database including human as well as 199 FBS protein sequences for MS data search of human cell secretomes. Searching against the human-FBS database returned more reliable results with fewer falsepositive and false-negative identifications compared to using either a human only database or a human-bovine database. Furthermore, the improved results validated our strategy without complex experiments like SILAC.We expect our strategy to improve the accuracy of human secreted protein identification and to also add value for general use.
URI
http://pubs.kist.re.kr/handle/201004/49862
ISSN
19326203
Appears in Collections:
KIST Publication > Article
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