Mass spectrometry-based N-linked glycomic profiling as a means for tracking pancreatic cancer metastasis
- Mass spectrometry-based N-linked glycomic profiling as a means for tracking pancreatic cancer metastasis
- Hae-Min Park; 황민태; Yoon-Woo Kim; Kyoung-Jin Kim; Jang Mi Jin; Young Hwan Kim; Yung-Hun Yang; 이관희; Yun-Gon Kim
- Pancreatic cells; N-glycans; Qualitative and quantitative analysis; MALDI-TOF MS
- Issue Date
- Carbohydrate research
- VOL 413, 5-11
- The aberrant glycosylation profile on the surface of cancer cells has been recognized for its potential diagnostic value towards assessing tumor progression. In this study, we initially investigate N-glycan profiles on the surface of normal (HPDE) and cancerous (Capan-1, Panc-1, and MIA PaCa-2) pancreatic cell lines, which are from different sites of pancreatic tumor. The enzymatically deglycosylated total N-glycans are permethylated via a quantitative solid-phase method and then analyzed by using MALDI-TOF MS and MALDI-QIT-TOF MS. We demonstrate that the level of high-mannose type glycans is higher among Capan-1 cells—pancreatic cancer cells that have metastasized to the liver—than that observed among Panc-1 and MIA PaCa-2 cells—pancreatic cancer cells from the pancreas duct head and tail regions, respectively. Furthermore, the relative abundance of highly-branched sialyted N-glycans is significantly up-regulated on Panc-1 and MIA PaCa-2 pancreatic cancer cells compared to that of normal HPDE pancreas cells. Taken together, these results indicate that specific N-glycosylation profile changes in pancreatic cancer cells can be used to not only distinguish between normal and cancerous cells but also provide more information on their location and metastatic potential.
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