Feasibility of a facile butanol bioproduction using planetary mill pretreatment

Title
Feasibility of a facile butanol bioproduction using planetary mill pretreatment
Authors
Jeong Heo KwonHyunsoo KangByoung-In Sang김연제Jiho MinRobert J. MitchellJin Hyung Lee
Keywords
Planetary mill; Pretreatment; Enhanced bioconversion; Biobutanol; Biofuel
Issue Date
2015-10
Publisher
Bioresource technology
Citation
VOL 게재예정, 게재예
Abstract
A facile butanol bioproduction process was developed using planetary milling, and Pinus rigida wood waste as a model substrate for fermentable sugars. The use of planetary milling as the pretreatment eliminates the need for washing and transfer of the biomass prior to enzymatic hydrolysis. Moreover, using this pretreatment process resulted in the production of only 0.072 ± 0.003 g/L soluble phenolic compounds, a concentration that was not inhibitory towards Clostridium beijerinckii NCIMB 8052. As the milling was performed in a compatible buffer (50 mM acetate, pH 4.8), the enzymatic hydrolysis step was initiated by simply adding the cellulase cocktail powder directly to pretreated biomass without washing the biomass or exchanging the buffer, resulting in a glucose yield of 31 g/L (84.02%). Fermentation of the hydrolysate samples by C. beijerinckii NCIMB 8052 gave slightly better butanol yields than cultures grown in a typical lab media (P2), with final concentrations of 6.91and 6.66 g/L, respectively
URI
http://pubs.kist.re.kr/handle/201004/50270
ISSN
09608524
Appears in Collections:
KIST Publication > Article
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