Hair sterol signatures coupled to multivariate data analysis reveal an increased 7b-hydroxycholesterol production in cognitive impairment
- Hair sterol signatures coupled to multivariate data analysis reveal an increased 7b-hydroxycholesterol production in cognitive impairment
- 손현화; 이도엽; 서홍석; 정지현; 문주연; 이정은; 정봉철; 김어수; 최만호
- Alzheimer; cholesterol
- Issue Date
- The Journal of steroid biochemistry and molecular biology
- VOL 155, 9-17
- Altered cholesterol metabolism could be associated with cognitive impairment. The quantitative profiling of 19 hair sterols was developed using gas chromatography–mass spectrometry coupled to multivariate data analysis. The limit of quantification of all sterols ranged from 5 to 20 ng/g, while the calibration linearity was higher than 0.98. The precision (% CV) and accuracy (% bias) ranged from 3.2% to 9.8% and from 83.2% to 119.4%, respectively. Among the sterols examined, 8 were quantitatively detected from two strands of 3-cm-long scalp hair samples of female participants, including mild cognitive impairment (MCI, n = 15), Alzheimer’s disease (AD, n = 31), and healthy controls (HC, n = 36). The cognitive impairment (MCI or AD) was correlated with a higher metabolic rate than that of HCs based on 7β-hydroxycholesterol (P < 0.005). Significant negative correlations (r = −0.822) were detected between Mini-Mental State Examination (MMSE) scores and hair sample metabolic ratios of 7β-hydroxycholesterol to cholesterol, which is an accepted, sensitive, and specific tool for discriminating HCs from individuals with MCI or AD. In conclusion, improved diagnostic values can be obtained using hair sterol signatures coupled with MMSE scores. This method may prove useful for predictive diagnosis in population screening of cognitive impairment.
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