Effects of phenolic acid metabolites formed after chlorogenic acid consumption on retinal degeneration in vivo
- Effects of phenolic acid metabolites formed after chlorogenic acid consumption on retinal degeneration in vivo
- 장호림; 최용수; 안홍열; 정상훈; 이창용
- Coffee metabolites; Chlorogenic acid; Hypoxia; Neuroprotection; Retinal degeneration
- Issue Date
- Molecular nutrition & food research
- VOL 59, 1918-1929
- Scope: Although ingestion of coffee and its constituent chlorogenic acid (CGA) protects the
retina from oxidative stress, the bioaccessibility and bioavailability of coffeemetabolites are not
well understood. The aim of this study was to determine which coffee metabolites reach the
retina and protect against retinal degeneration.
Methods and results: UPLC-MS/MS was used to detect CGA and coffee metabolites in the rat
eye. The methyl thiazolyl tetrazolium assay and double staining with Hoechst and propidium
iodide showed that CGA, caffeic acid (CA), and dihydrocaffeic acid (DHCA) protect retinal
ganglion cells from hypoxia-induced damage. Western blots showed that treatment with coffee
metabolites up-regulated anti-apoptotic proteins such as Bcl-2 and Bcl-XL and down-regulated
pro-apoptotic proteins such as Bad, PARP, and cleaved caspase 3.Adult ICRmice were subjected
to optic nerve crush-induced retinal ganglion cell death with intravitreal pre-treatment with
coffee metabolites 1 day before and 1 h after the procedure. Retrograde FluorogoldTM labeling
showed severe retinal ganglion cell loss after optic nerve crushing, and coffee metabolites
significantly reduced damage to retinal ganglion cells.
Conclusion: CGA and coffeemetabolites, especially, CA, and DHCA, reach the eye, where they
can significantly reduce apoptosis induced by hypoxia and optic nerve crush stress, and thus
prevent retinal degeneration.
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