Effects of phenolic acid metabolites formed after chlorogenic acid consumption on retinal degeneration in vivo

Title
Effects of phenolic acid metabolites formed after chlorogenic acid consumption on retinal degeneration in vivo
Authors
장호림최용수안홍열정상훈이창용
Keywords
Coffee metabolites; Chlorogenic acid; Hypoxia; Neuroprotection; Retinal degeneration
Issue Date
2015-10
Publisher
Molecular nutrition & food research
Citation
VOL 59, 1918-1929
Abstract
Scope: Although ingestion of coffee and its constituent chlorogenic acid (CGA) protects the retina from oxidative stress, the bioaccessibility and bioavailability of coffeemetabolites are not well understood. The aim of this study was to determine which coffee metabolites reach the retina and protect against retinal degeneration. Methods and results: UPLC-MS/MS was used to detect CGA and coffee metabolites in the rat eye. The methyl thiazolyl tetrazolium assay and double staining with Hoechst and propidium iodide showed that CGA, caffeic acid (CA), and dihydrocaffeic acid (DHCA) protect retinal ganglion cells from hypoxia-induced damage. Western blots showed that treatment with coffee metabolites up-regulated anti-apoptotic proteins such as Bcl-2 and Bcl-XL and down-regulated pro-apoptotic proteins such as Bad, PARP, and cleaved caspase 3.Adult ICRmice were subjected to optic nerve crush-induced retinal ganglion cell death with intravitreal pre-treatment with coffee metabolites 1 day before and 1 h after the procedure. Retrograde FluorogoldTM labeling showed severe retinal ganglion cell loss after optic nerve crushing, and coffee metabolites significantly reduced damage to retinal ganglion cells. Conclusion: CGA and coffeemetabolites, especially, CA, and DHCA, reach the eye, where they can significantly reduce apoptosis induced by hypoxia and optic nerve crush stress, and thus prevent retinal degeneration.
URI
http://pubs.kist.re.kr/handle/201004/50367
ISSN
16134125
Appears in Collections:
KIST Publication > Article
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