Coculture of Primary Motor Neurons and Schwann Cells as a Model for In Vitro Myelination
- Coculture of Primary Motor Neurons and Schwann Cells as a Model for In Vitro Myelination
- 형수진; 이보윤; 박종철; 김진석; 허은미; 서준교
- Issue Date
- Scientific Reports
- VOL 5
- A culture system that can recapitulate myelination in vitro will not only help us better understand
the mechanism of myelination and demyelination, but also find out possible therapeutic
interventions for treating demyelinating diseases. Here, we introduce a simple and reproducible
myelination culture system using mouse motor neurons (MNs) and Schwann cells (SCs). Dissociated
motor neurons are plated on a feeder layer of SCs, which interact with and wrap around the axons
of MNs as they differentiate in culture. In our MN-SC coculture system, MNs survived over 3 weeks
and extended long axons. Both viability and axon growth of MNs in the coculture were markedly
enhanced as compared to those of MN monoculture. Co-labeling of myelin basic proteins (MBPs) and
neuronal microtubules revealed that SC formed myelin sheaths by wrapping around the axons of
MNs. Furthermore, using the coculture system we found that treatment of an antioxidant substance coenzyme Q10 (Co-Q10) markedly facilitated myelination.
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