Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels
- Scaffold-free, Human Mesenchymal Stem Cell-Based Tissue Engineered Blood Vessels
- 정영미; 지하연; Zaozao Chen; Hon Fai Chan; Leigh Atchison; Bruce Klitzman; George Truskey; Kam W. Leong
- Issue Date
- Scientific Reports
- VOL 5, 15116-1-15116-9
- Tissue-engineered blood vessels (TEBV) can serve as vascular grafts and may also play an important role in the development of organs-on-a-chip. Most TEBV construction involves scaffolding with biomaterials such as collagen gel or electrospun fibrous mesh. Hypothesizing that a scaffold-free TEBV may be advantageous, we constructed a tubular structure (1mm i.d.) from aligned human mesenchymal cell sheets (hMSC) as the wall and human endothelial progenitor cell (hEPC) coating as the lumen. The burst pressure of the scaffold-free TEBV was above 200mmHg after three weeks of sequential culture in a rotating wall bioreactor and perfusion at 6.8 dynes/cm2 . The interwoven organization of the cell layers and extensive extracellular matrix (ECM) formation of the hMSCbased TEBV resembled that of native blood vessels. The TEBV exhibited flow-mediated vasodilation, vasoconstriction after exposure to 1μM phenylephrine and released nitric oxide in a manner similar to that of porcine femoral vein. HL-60 cells attached to the TEBV lumen after TNF-α activation to suggest a functional endothelium. This study demonstrates the potential of a hEPC endothelialized hMSC-based TEBV for drug screening.
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