Characterization of the efficient cellulase in an isolated cellulolytic bacterium Paenibacillus sp. CAA11
- Characterization of the efficient cellulase in an isolated cellulolytic bacterium Paenibacillus sp. CAA11
- 조숙형; 김은숙; 우한민; 엄영순
- Issue Date
- 한국생물공학회 창립 30주년 기념
학술발표대회 및 국제심포지엄
- Cellulosic biomass is the most abundant and attractive resource for biorefinery that aims to produce valuable bio-chemicals. In this study, we investigated an isolated cellulolytic bacterium, designated Paenibacillus sp. CAA11 from soil and its cellulase. The isolate was able to grow using carboxymethyl cellulose (CMC) and secreted cellulase by own secretion system. To determine an effective cellulase in Paenibacillus sp. CAA11, putative genes encoding cellulase in CAA11 were cloned and overexpressed in Bacillus subtilis RIK1285 which is deficient in two kinds of proteases. After Congo-red staining, the recombinant B. subtilis RIK1285 overexpressed CAA11_02340 showed 2.1-fold bigger halo zone on CMC containing plate than RIK1285 wild type, indicating that putative cellulase CAA11_02340 was functional to degrade cellulose. Furthermore, B. subtilis RIK1285 overexpressed CAA11_02340 was able to degrade insoluble regenerated amorphous cellulose. These results suggest that the recombinant B. subtilis RIK1285 containing CAA11_02340 could be used as a consolidated bioprocessing platform after metabolic pathway engineering toward a cost-effective biorefinery using cellulosic biomass.
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