Development of a sensitive SPR biosensor for C-reactive protein (CRP) using plasma-treated parylene-N film
- Development of a sensitive SPR biosensor for C-reactive protein (CRP) using plasma-treated parylene-N film
- Yong-Hwan Choi; Hyuk Ko; Ga-Yeon Lee; Seo-Yoon Chang; Young Wook Chang; 강민정; Jae-Chul Pyun
- Parylene-N; Surface modification; Plasma; Immobilization; SPR biosensor
- Issue Date
- Sensors and actuators. B, Chemical
- VOL 207, NO pt.A, 133-138
- Parylene-N film has high protein immobilization property after plasma-treatment. In this work, the optimum conditions for plasma-treatment of parylene-N films were investigated to develop a highly sensitive SPR biosensor. The effects of the plasma power and plasma-treatment time on the surface of parylene-N films were analyzed by measuring film contact angle and roughness from atomic force microscopy images. The plasma power was applied at a range of 0–100 W for different treatment times. The influence of plasma-treatment was also analyzed using the depth profiles from X-ray photospectroscopy. From this analysis, chemical functional groups (those related to oxygen) were introduced and were estimated to penetrate up to 4 nm and 8 nm from the top surface of the parylene-N film due to treatment with plasma powers of 50 W and 100 W, respectively, for 1 min. Based on the optimum plasma-treatment conditions for the plasma power and the plasma-treatment time, an SPR biosensor was developed for the detection of the human C-reactive protein.
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