Conventional and Improved Cytotoxicity Test Methods of Newly Developed Biodegradable Magnesium Alloys

Title
Conventional and Improved Cytotoxicity Test Methods of Newly Developed Biodegradable Magnesium Alloys
Authors
한형섭김희경김유찬석현광김영율
Keywords
biomaterials; biocompatibility; corrosion; cytotoxicity; metals
Issue Date
2015-11
Publisher
Metals and Materials International
Citation
VOL 21, NO 6, 1108-1117
Abstract
Unique biodegradable property of magnesium has spawned countless studies to develop ideal biodegradable orthopedic implant materials in the last decade. However, due to the rapid pH change and extensive amount of hydrogen gas generated during biocorrosion, it is extremely difficult to determine the accurate cytotoxicity of newly developed magnesium alloys using the existing methods. Herein, we report a new method to accurately determine the cytotoxicity of magnesium alloys with varying corrosion rate while taking in-vivo condition into the consideration. For conventional method, extract quantities of each metal ion were determined using ICP-MS and the result showed that the cytotoxicity due to pH change caused by corrosion affected the cell viability rather than the intrinsic cytotoxicity of magnesium alloy. In physiological environment, pH is regulated and adjusted within normal pH (~7.4) range by homeostasis. Two new methods using pH buffered extracts were proposed and performed to show that environmental buffering effect of pH, dilution of the extract, and the regulation of eluate surface area must be taken into consideration for accurate cytotoxicity measurement of biodegradable magnesium alloys.
URI
http://pubs.kist.re.kr/handle/201004/50553
ISSN
15989623
Appears in Collections:
KIST Publication > Article
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