Enhanced Cytoplasmic Delivery of RAGE siRNA Using Bioreducible Polyethylenimine-based Nanocarriers for Myocardial Gene Therapy

Title
Enhanced Cytoplasmic Delivery of RAGE siRNA Using Bioreducible Polyethylenimine-based Nanocarriers for Myocardial Gene Therapy
Authors
양민정구숙희김동규Won Jong KimHyejung Mok김선화권익찬
Keywords
bioreducible polyethylenimine; cytoplasmic delivery; myocardial infarction; RAGE; siRNA
Issue Date
2015
Publisher
Macromolecular bioscience
Citation
VOL 게재예정, 게재예
Abstract
This study aims to develop bioreducible polyethylenimine (rPEI)/siRNA polyplexes with high stability, high transfection efficiency, and low cytotoxicity for efficient cytoplasmic siRNA delivery. rPEI successfully incorporated siRNA into stable and compact nanocomplexes, and the disulfide linkages in rPEI/siRNA were cleaved under reductive environments, resulting in efficient intracellular translocation and siRNA release. In this study, receptor for advanced glycation end-products (RAGE) was selected as a therapeutic target gene because it is associated with inflammatory responses in ischemia/reperfusion injury. rPEI/siRAGE exhibited high target gene silencing and low cytotoxicity in cardiomyocytes, and the treatment of rPEI/siRAGE reduced the myocardial infarction size
URI
http://pubs.kist.re.kr/handle/201004/50570
ISSN
16165187
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KIST Publication > Article
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